Veripath Test Directory

Epic Test Name FIBRINOGEN, Antigen
Synonyms Fibrinogen Antigen
Description Fibrinogen is soluble plasma protein that is polymerized to insoluble fibrin by the action of thrombin resulting in the formation of a fibrin clot. In this assay, immunological method is used to measure fibrinogen concentration rather than the functional activity. This assay is useful in the investigation of congenital dysfibrinogenemia where there is a discrepancy between the antigen level and the functional activity.
Specimen Requirements 
Container Light blue (3.2% sodium citrate) collection tube
Specimen Type Plasma
Preferred Collection Volume 3 mL platelet-poor citrated plasma
Minimum Collection Volume Adult: 2 mL, Neonates : 2 mL
Collection Instructions When drawing specimens for coagulation testing, always draw a "pilot" tube (7-10ml plain red top tube), completely filled. This removes tissue fluid contamination. Samples should be obtained from peripheral vein wheneever possible. If drawn from indwelling catheter, the line should be flushed with 15mL of saline. After irrigation, a "pilot" tube should be drawn and discarded.
Specimen Handling In-house patients, transport at room temperature.
Out-side client, follow collection instructions and the steps below:
1. Spin light blue top tube. Remove plasma avoiding platelet/buffy coat layer. Spin plasma again to remove top portion of plasma ans place 1mL of PLATELET-POOR PLASMA in plastic tube. (Glass tube unacceptable). Double-centrifugation is critical for accurate results as platelet contamination may cause erroneous results.
2. Plasma must be frozen within 4 hours of collection. CRITICAL WARNING: Specimens must remain frozen until ready to test. Submit separate aliquots when multiple tests are ordered on the same patient specimen.
Rejection Criteria Hemolysis, clotted, short sample, >4 hrs old (if not frozen), thawed plasma, heparin contamination.
Methodology Immune - turbometric
Reference Range 149 - 353 mg/dl
CPT Code(s) 85385
Department Hemostasis/Coagulation
Contact Information Hemostasis Lab; 214-633-4959 Customer Service: 214-633-0400, Toll Free 1-877-887-8136.
Turn Around Time1·3 days
Performed Monday - Friday
Performed by Hemostasis
Updated/Reviewed 07/25/2016