Veripath Test Directory

Epic Test Name Collagen Binding Assay
Description The Collagen Binding Assay is an enzyne immunoassay for the qualitative determination of vonWillebrand Factor (vWF) function in human plasma. The Collagen Binding Assay (CBA) can be used to differentiate between von Willebrand Disorder (vWD) Type I and Type II when used in conjunction with the vWF antigen (vWF:Ag) assay. This is an ELISA based assay.
Specimen Requirements 
Container Light blue (3.2% sodium citrate) collection tube
Specimen Type Blood
Preferred Collection Volume 3 mL
Minimum Collection Volume Adult: 2 mL, Neonates : 2 mL
Collection Instructions When drawing specimens for coagulation testing, always draw a "pilot" tube (7-10ml plain red top tube), completely filled. This removes tissue fluid contamination. Samples should be obtained from peripheral vein whenever possible. If drawn from indwelling catheter, the line should be flushed with 15mL of saline. After irrigation, a "pilot" tube should be drawn and discarded.
Specimen Handling In-house patients, transport at room temperature.
Out-side client, follow collection instructions and the steps below:
1. Spin light blue top tube. Remove plasma avoiding platelet/buffy coat layer. Spin plasma again to remove top portion of plasma ans place 1mL of PLATELET-POOR PLASMA in plastic tube. (Glass tube unacceptable). Double-centrifugation is critical for accurate results as platelet contamination may cause erroneous results.
2. Plasma must be frozen within 4 hours of collection. CRITICAL WARNING: Specimens must remain frozen until ready to test. Submit separate aliquots when multiple tests are ordered on the same patient specimen.
Rejection Criteria Hemolysis, clotted, short sample, >4 hrs old (if not frozen), thawed plasma, heparin contamination
Methodology ELISA
Reference Range 50 - 97%
CPT Code(s) 85245
Department Hemostasis/Coagulation
Contact Information Hemostasis Lab; 214-633-4959 Customer Service: 214-633-0400, Toll Free 1-877-887-8136
Turn Around Time1-7 days
Performed Friday or as needed
Performed by Hemostasis Lab
Updated/Reviewed 07/23/2016