Veripath Test Directory

Epic Test Name IMMUNOPHENOTYPING FOR LEUKEMIAS AND LYMPHOMAS
Components
Synonyms Lymphoma Immunophenotyping; Flow Cytometry - Tissue, Fluid, Bone Marrow, Blood, Lymphoproliferative Disorder Tissue Panel, Leukemia Immunophenotyping
Description A UTSW hematopathologist will triage a specimen using clinical information in conjunction with a morphologic examination to determine an appropriate immunophenotypic panel to be performed. This panel is a carefully considered combination of antibodies that allows for accurate assessment of cell populations within a specimen. While each panel is designed for optimal characterization of specific cell populations in the sample (for example, a lymph node specimen is assessed with a panel that focuses primarily on T, NK and B Lymphocytes), every panel is designed to identify any unexpected cell populations that may be present. If needed, additional custom markers are added to completely characterize any and all abnormal cell populations. This approach greatly increases both the sensitivity and specificity of the flow cytometry analysis. An interpretation of the immunophenotypic findings and correlation with the morphologic features is provided by a UTSW hematopathologist.

The price of each panel is based on the number and type of markers used. If an abnormality is defined but not fully analyzed in the initial panel, additional markers will be added, only as necessary, to confirm and characterize the aberrant population.
Specimen Requirements 
Container Yellow (ACD A or B), Lavender (EDTA), Green (NA Heparin) tubes, sterile cup, syringe or transport tube
Specimen Type Bone Marrow; Peripheral Blood; Solid Tissue; Body fluids; Cerebrospinal fluid
Preferred Collection Volume Bone Marrow - 3ML; Peripheral Blood - 10mL; Solid tissue - As much as possible; Body Fluids - At least 5 mL; CSF - As much as possible.
Minimum Collection Volume
Collection Instructions Specimens cannot be frozen or placed in any fixative (i.e. formalin). Bone marrow collected in a heparinized syringe should be sent to the laboratory immediately. Transport bone marrow and peripheral blood at room temperature. EDTA and Na Heparin specimens should be processed within 48 hours of collection. ACD specimens should be processed within 72 hours of collection. Solid tissue, body fluids and CSF should be refrigerated at 2-8° C. Transport CSF immediately.
Specimen Handling Specimen cannot be frozen or placed in any fixative (i.e. formalin).
To process the specimen, the request form must include:
*Collection date & time.
*Pertinent clinical history (with relevant ICD-9 codes, if available)
*Specimen source (fluid type, tissue origin)
*Patient identification (i.e. name, DOB, MR#).
*Physician requesting test.
Rejection Criteria Specimens cannot be frozen or placed in any fixative (i.e. formalin).
Methodology Flow Cytometry
Reference Range
CPT Code(s) 88184, 88185 x (number of markers)
and 88187 (2-8 markers)
or 88188 (9-15 markers)
or 88189 (16 and up markers)
Department Flow Cytometry
Contact Information 214-648-0930 or 214-633-5227
Turn Around TimeResults reported by the close of the next business day. STAT service on holidays, weekends and after business hours is available with direct coordination with the laboratory staff (additional charges may apply).
Performed Monday - Saturday
Performed by Flow Cytometry
Link
Notes Process & Hold: the bone marrow, blood or tissue specimens will be processed and retained for up to 3 days. Mark "Process & Hold" on the requisition form and contact the lab as soon as possible with further instructions (i.e., whether or not to run). A processing fee may apply.
Updated/Reviewed 11/06/2018


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