Veripath Test Directory

Epic Test Name Lupus Anticoagulant
Components Dilute Russel Venom Time - screen, Dilute Russel Venom Time - confirmation, Dilute Russel Venom Time - ratio, Silica Clotting Time - screen, Silica Clotting Time - confirmation, Silica Clotting Time - ratio.
Synonyms LA
Description Lupus anticoagulants (LA) are a heterogeneous group of antibodies directed against plasma proteins, which also bind to phospholipid surfaces. Often, there are no clinical consequences other than the need to explain the reason for the long aPTT. A minority of patients with LA have a hypercoagulable state manifested by recurrent thromboses, multiple spontaneous miscarriages, migraineheadaches, or stroke. Very rarely, patients may have bleeding.
Two screening test (Dilute Russel Venom Time and Silica Clotting Time) utilized simultaneously to screen for Lupus anticoagulant. Positive screening test is then confirmed by confirmatory test.
Specimen Requirements 
Container Light blue (3.2 % sodium citrate) collection tube
Specimen Type Blood
Preferred Collection Volume 3 mL
Minimum Collection Volume Adult: 2 mL, Neonates : 2 mL
Collection Instructions When drawing specimens for coagulation testing, always draw a "pilot" tube (7-10ml plain red top tube), completely filled. This removes tissue fluid contamination. Samples should be obtained from peripheral vein whenever possible. If drawn from indwelling catheter, the line should be flushed with 15mL of saline. After irrigation, a "pilot" tube should be drawn and discarded.
Specimen Handling In-house patients, transport at room temperature.
Out-side client, follow collection instructions and the steps below:
1. Spin light blue top tube. Remove plasma avoiding platelet/buffy coat layer. Spin plasma again to remove top portion of plasma ans place 1mL of PLATELET-POOR PLASMA in plastic tube. (Glass tube unacceptable). Double-centrifugation is critical for accurate results as platelet contamination may cause erroneous results.
2. Plasma must be frozen within 4 hours of collection. CRITICAL WARNING: Specimens must remain frozen until ready to test. Submit separate aliquots when multiple tests are ordered on the same patient specimen.
Rejection Criteria Hemolysis, clotted, short sample, >4 hrs old (if not frozen), thawed plasma, heparin contamination.
Methodology Clotting based.
Reference Range See interpretive report.
CPT Code(s)
Department Hemostasis/Coagulation
Contact Information Hemostasis Lab; 214-633-4959 Customer Service: 214-633-0400, Toll Free 1-877-887-8136
Turn Around Time3-5 days
Performed Wednesday and Friday
Performed by Hemostasis Lab
Updated/Reviewed 07/25/2016