Veripath Test Directory

Epic Test Name FISH, Pediatric Acute Myeloid Leukemia (AML) Panel
Synonyms Pediatric AML FISH Panel
Pediatric Acute Myeloid leukemia FISH Panel
Description Fluorescence In Situ Hybridization is performed to detect chromosome 5 aberrations, chromosome 7 aberrations, RUNX1T1/RUNX1, BCR/ABL1, MLL, PML/RARA and CBFB gene rearrangements.
Specimen Requirements 
Container Green top (Sodium heparin) collection tube.
Specimen Type Bone marrow, blood, tissue.
Preferred Collection Volume 2 mL bone marrow, 5-10 mL blood, 5mm3 tissue.
Minimum Collection Volume
Collection Instructions Bone Marrow: 2 mL aspirated bone marrow preferred) anticoagulated with preservative-free sodium heparin (e.g., greenĀ¬ top tube). DO NOT use lithium heparin. Bone marrow biopsy may be submitted if aspirate is not available - see instructions for
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. Frozen or fixed tissue specimens, or tissue specimens sent without tissue culture medium.
Methodology See methodology description for each FISH test.
Reference Range See interpretive report
CPT Code(s) 88271x14, 88275X7, 88291. If routine Cytogenetics is not requested, add 88237 (bone marrow/blood) or 88239 (tumor).
Department Cytogenetics
Contact Information 214-648-0975 or (214) 645-7057 or Toll free: (877)-887-8136
Turn Around Time1-3 days
Performed Sunday-Saturday
Performed by Cytogenetics
Updated/Reviewed 12/12/2016