Veripath Test Directory

Epic Test Name FISH, CRI-DU-CHAT SYNDROME MICRODELETION
Components
Synonyms FISH, 5p- Microdeletion
Microdeletion FISH
Cri-du-chat 5p- FISH
Description Fluorescence In Situ Hybridization (FISH) is performed to detect microdeletions of the cri-du-chat syndrome critical region on the short arm of chromosome 5.
Specimen Requirements 
Container Green top (Sodium heparin) collection tube.
Specimen Type Blood
Preferred Collection Volume 3 mL
Minimum Collection Volume 2 mL
Collection Instructions Minimum of 3 Ml asceptically collected blood in 5 mL green top, sodium heparin tube or ( 2 mL pediatric green top, sodium heparin tube). DO NOT use lithium heparin tubes.
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used
Methodology A fluorescent labeled DNA probes , cri-du-chat region/SOTOS region at chromosome band 5p15.2 is hybridized to unstained interphase chromosomes. Ten cells are evaluated for the presence of 2 signals (normal) or 1 signal (deletion).
Reference Range See interpretive report.
CPT Code(s) 88271, 88273, 88291 (if routine Cytogenetics not requested, add 88230).
Department Cytogenetics
Contact Information Lab: (214) 648-0975, Customer Service:(214) 645-7057 or Toll free: (877)- 887-8136
Turn Around Time7-10 DAYS
Performed Sunday - Saturday
Performed by Cytogenetics
Link
Notes "Cytogenetics, Blood, Constitutional Karyotype" must be ordered on the same specimen, unless Cytogenetics analysis has been performed on a prior specimen.
Updated/Reviewed 12/12/2016


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