Veripath Test Directory

Epic Test Name FISH, EGFR
Synonyms EGFR FISH
Description Fluorescence in situ hybridization (FISH) is performed to detect the EGFR gene amplification, most commonly observed in lung cancer.
Specimen Requirements 
Container Two unstained positively charged slides cut at 4 microns and one H&E stain slide
Specimen Type FFPE (Formalin Fixed Paraffin Embedded Tissue)
Preferred Collection Volume
Minimum Collection Volume
Collection Instructions
Specimen Handling
Rejection Criteria Cases without H&E slide.
Methodology A dual color fluorescent labeled DNA probe for the EGFR gene, at chromosome band 7p12, is hybridized to unstained interphase nuclei on a slide. 200 cells are evaluated to determine whether the signals are amplified.
Reference Range See interpretive report.
CPT Code(s) 88271x2,88275,88291
Department Cytogenetics
Contact Information Cytogenetics Lab: (214) 648-0975, Customer Service: (214) 645-7057 or Toll Free (877) 887-8136
Turn Around Time6 working days after specimen arrival in Cytogenetics Lab
Performed 3 times a week
Performed by Cytogenetics
Updated/Reviewed 12/12/2016