Veripath Test Directory

Synonyms FGFR3/IGH gene rearrangement by FISH
t(4;14) FISH
Description Fluorescence In situ hybridization (FISH) is performed to detect the (4;14) translocation or other 14q32 rearrangements that are characteristic of multiple myeloma.
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Bone marrow, blood, tissue
Preferred Collection Volume 2 mL bone marrow, 5-10 ml blood, 5mm³ tissue
Minimum Collection Volume
Collection Instructions 1. Bone Marrow: 2 mL aspirated bone marrow preferred) anticoagulated with preservative-free sodium heparin (e.g., green­ top tube). DO NOT use lithium heparin. Bone marrow biopsy may be submitted if aspirate is not available - see instructions for
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. Frozen or fixed tissue specimens, or tissue specimens sent without tissue culture medium.
Methodology Dual color, dual fusion fluorescent labeled DNA probes for the FGFR3 gene at chromosome band 4p16 and the IGH gene at chromosome band 14q32, are hybridized to unstained interphase nuclei on a slide. 200 cells are evaluated to determine If the signals are discreet (no rearrangement) or fused (rearrangement).
Reference Range
CPT Code(s) 88271x2; 88275; 88291
Department Cytogenetics
Contact Information Lab: (214) 648-0975, Customer Service:(214) 645-7057 or Toll free: (877)- 887-8136
Turn Around Time1-3 Days
Performed Sunday - Saturday
Performed by Cytogenetics
Notes See also: Multiple Myeloma FISH panel

Conventional cytogenetic analysis should be performed In addition to FISH at Initial diagnosis.
Updated/Reviewed 12/12/2016