Veripath Test Directory

Description Autoimmune lymphoproliferative syndrome (ALPS) is a primary immunodeficiency disorder due to defective FAS mediated apoptosis of lymphocytes. This defect leads to chronic non-malignant lymphoproliferation, autoimmune disease and an increased risk of lymphoma. Screening for ALPS is performed by flow cytometry on peripheral blood. This test assesses for an elevation in the CD3(+)/TCRαβ(+)/CD4(-)/ CD8(-) subset of T cells. This information is used in conjunction with clinical information and other laboratory data to establish a preliminary diagnosis of ALPS.
Specimen Requirements 
Container 1-10mL yellow (ACD-A) or (ACD- B), 1 - 5mL lavender (K2-K3 EDTA), or 1-5mL green (Na Heparin)
Specimen Type Peripheral Blood
Preferred Collection Volume 10mL
Minimum Collection Volume 5mL Yellow (ACD-A) or (ACD- B), 2 mL EDTA, 2 mL Na Heparin
Collection Instructions EDTA specimens should be processed within 48 hours of collection. ACD specimens should be processed within 72 hours of collection.
Specimen Handling Transport at room temperature (20 - 25⁰C).
Rejection Criteria All tubes must be properly drawn, partial draw may cause cell lysis and loss due to hypertonic osmotic changes. Clotted, underfilled, hemolyzed or frozen specimens will be rejected.
Methodology Flow Cytometry
Reference Range
CPT Code(s) 88184,88185 (x number of markers) and 88187 (2-8 markers) or 88188 (9-15 markers) or 88189 (16 or greater markers)
Department Flow Cytometry
Contact Information 214-648-0930 or 214-633-5227
Turn Around TimeSpecimens received before 5 PM Monday-Friday should be resulted by 6 PM the next business day.
Performed Monday - Saturday
Performed by Flow Cytometry
Updated/Reviewed 11/06/2018