Veripath Test Directory

Epic Test Name FISH, SRY
Synonyms SRY FISH
Male sex determination FISH
FISH, male sex determination
Description Fluorescence In Situ Hybridization (FISH) is performed to detect the presence or absence of the SRY gene (sex-determining region on the Y chromosome). Useful when karyotypic sex and phenotypic sex do not correspond.
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Blood
Preferred Collection Volume 3 mL
Minimum Collection Volume 2 mL
Collection Instructions Minimum 3 mL aseptically collected blood in a 5 mL green top, sodium heparin tube (or 2 mL pediatric green top, sodium heparin tube). DO NOT use lithium heparin tubes.
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated If not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. Frozen or fixed tissue specimens, or tissue specimens sent without tissue culture medium.
Methodology A fluorescent labeled DNA probe for the SRY gene on the short arm of the Y chromosome Is hybridized to unstained metaphase chromosomes. Ten cells are evaluated for the presence or absence of the target signal. Can also be performed on interphase nuclei.
Reference Range See interpretive report.
CPT Code(s) 88271, 88273,88291(if routine cytogenetics not requested, add 88230)Interphase method: 88271, 88275, 88291
Department Cytogenetics
Contact Information Cytogenetics Lab: ( 214) 648-0975, Customer Service: (214) 645-7057 or Toll Free (877) 887-8136
Turn Around Time7-10 days
Performed Sunday - Saturday
Performed by Cytogenetics
Notes Cytogenetics, Blood. Constitutional Karyotyoe must be ordered on the same specimen, unless cytogenetic analysts has been performed on a prior specimen.
Updated/Reviewed 12/12/2016