Veripath Test Directory

Synonyms FISH, chromosome 1 short arm microdeletion
1p- Microdeletion FISH
Description Fluorescence in Situ Hybridization (FISH) is performed to detect microdeletions of the terminal portion of the short arm of chromosome 1.
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Blood
Preferred Collection Volume 3 mL
Minimum Collection Volume 2 mL
Collection Instructions Minimum 3 mL aseptically collected blood In a 5 mL green top, sodium heparin tube (or 2 mL pediatric green top, sodium heparin tube). DO NOT use lithium heparin tubes.
Specimen Handling
Rejection Criteria Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used.
Methodology A fluorescent labeled DNA probe for the p58 gene, at chromosome band 1p36, is hybridized to unstained metaphase chromosomes. Ten cells are evaluated for the presence of 2 signals (normal) or 1 signal (deletion).
Reference Range See interpretive report.
CPT Code(s) 88271, 88273, 88291(if routine cytogenetics not requested, add 88230)
Department Cytogenetics
Contact Information Cytogenetics Lab: (214) 648-0975, Customer Service: (214) 645-7057 or Toll Free (877) 887-8136
Turn Around Time7-10 days
Performed Sunday - Saturday
Performed by Cytogenetics
Notes Cvtoqenetics, Blood, Constitutional Karyotype must be ordered on the same specimen, unless cytogenetic analysis has been performed on a prior specimen.
Updated/Reviewed 12/12/2016