Veripath Test Directory

Epic Test Name CHROMOSOME ANALYSIS, SOLID TISSUE, NON-NEOPLASTIC
Components
Synonyms Cytogenetics, Solid Tissue, Non-Neoplastic
Karyotype, Solid Tissue, Non-Neoplastic
Description Cytogenetic analysis of cultured fibroblasts to assess for the presence of constitutional (congenital) chromosome aberrations.
Specimen Requirements 
Container
Specimen Type
Preferred Collection Volume 5mm³ of aseptically collected fresh tissue from surgery or autopsy, or a generous 2 mm diameter (minimum) full thickness skin biopsy.
Minimum Collection Volume
Collection Instructions Do not send skin from autopsy. Kidney, lung, fascia, cartilage and chorionic villi are preferred specimens from autopsy. Place specimen in 10 mL of sterile tissue culture medium and refrigerate until transported. Do not freeze. Do not put in formalin
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, fixed or grossly contaminated specimens. Specimens sent without tissue culture medium.
Methodology Tissue is minced and/or enzymatically disaggregated and is cultured until sufficient dividing cells are present (10 days to 4 weeks). Standard cytogenetic harvest. G-banding with trypsin-Wright stain. The chromosomes in twenty metaphase spreads are counted, five cells are fully analyzed and two are karyotyped.
Reference Range See interpretive report.
CPT Code(s) 88233, 88262, 88291
Department Cytogenetics
Contact Information Cytogenetics Lab: (214) 648-0975, Customer Service:(214) 645-7057 or Toll Free (877) 887-8136
Turn Around Time2-3 weeks (dependent on growth rate of cultured cells).
Performed Sunday - Saturday
Performed by Cytogenetics
Link
Notes
Updated/Reviewed 12/12/2016


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