Veripath Test Directory

Epic Test Name FISH, BCL6
Components
Synonyms Diffuse large B-cell lymphoma FISH
DLBCL FISH
BCL6 FISH
Description Fluorescence In situ hybridization (FISH} Is performed to detect rearrangements of the BCL6 gene most commonly associated with diffuse large B-cell lymphoma.
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Bone marrow, blood, tissue
Preferred Collection Volume 2 mL bone marrow, 5-10 mL blood, 5mm³ tissue
Minimum Collection Volume
Collection Instructions 1. Bone Marrow: 2 mL aspirated bone marrow preferred) anticoagulated with preservative-free sodium heparin (e.g., green­ top tube). DO NOT use lithium heparin. Bone marrow biopsy may be submitted if aspirate is not available - see instructions for
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, clotted,hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. Frozen or fixed tissue specimens, or tissue specimens sent without tissue culture medium.
Methodology A dual color fluorescent labeled DNA probe for the BCL6 gene, at chromosome band 3q27, Is hybridized to unstained Interphase nuclei on a slide. 200 cells are evaluated to determine whether the signals are fused (normal) or separated (rearrangement present).
Reference Range See interpretive report.
CPT Code(s) 88271 x 2, 88275, 88291
Department Cytogenetics
Contact Information Lab: (214) 648-0975, Customer Service: (214) 645-7057 or Toll free: (877)-887-8136
Turn Around Time1-3 days
Performed Sunday - Saturday
Performed by Cytogenetics
Link
Notes Conventional cytogenetic analysis should be performed in addition to FISH at initial diagnosis
Updated/Reviewed 12/12/2016


Label