Veripath Test Directory

Epic Test Name FISH, ATM
Synonyms ATM FISH
CLL FISH panel
Description Fluorescent in situ hybridization (FISH) is performed to detect deletion of the ATM gene as seen in chronic lymphocytic lukemia (CLL).
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Bone marrow, blood, tissue
Preferred Collection Volume 2 mL bone marrow, 5-10 mL blood, 5mm³ tissue
Minimum Collection Volume
Collection Instructions 1. Bone Marrow: 2 mL aspirated bone marrow preferred) anticoagulated with preservative-free sodium heparin (e.g., green­ top tube). DO NOT use lithium heparin. Bone marrow biopsy may be submitted if aspirate is not available - see instructions for t
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria
Methodology A fluorescent labeled DNA probe for the ATM gene, at chromosome band 11q22.3, is hybridized to unstained interphase nuclei on a slide. 200 cells are evaluated to determine whether two signals are present (no deletion) or fewer than two signals are present (deletion).
Reference Range See interpretive report
CPT Code(s) 88271, 88275, 88291
Department Cytogenetics
Contact Information Lab: (214) 648-0975, Customer Service:(214) 645-7057 or Toll free: (877)-887-8136
Turn Around Time1-3 Days
Performed Sunday - Saturday
Performed by Cytogenetics
Notes Conventional cytogenetic analysis should be performed in addition to FISH at initial diagnosis
Updated/Reviewed 12/12/2016