Veripath Test Directory

Synonyms ABL1 FISH
Description Fluorescence in situ hybridization (FISH) is performed to detect amplification of the ABL1 gene as seen in precursor T-cell acute lymphoblastic leukemia.
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Bone marrow, blood or tissue
Preferred Collection Volume 2 mL bone marrow, 5-10 mL blood, 5mm³ tissue
Minimum Collection Volume
Collection Instructions 1. Bone Marrow: 2 mL aspirated bone marrow (preferred) anticoagulated with preservative-free sodium heparin (e.g., green­ top tube). DO NOT use lithium heparin. Bone marrow biopsy may be submitted if aspirate is not available - see instructions fo
Specimen Handling Deliver to lab at room temperature within 24 hours. f collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported Immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen,clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. .
Methodology A fluorescent labeled DNA probe for the ABL1 gene, at chromosome band
9q34, is hybridized to unstained interphase nuclei on a slide. 200 cells are evaluated to determine whether amplification is present.
Reference Range See interpretive report.
CPT Code(s) 88271, 88275, 88291
Department Cytogenetics
Contact Information Lab: (214) 648-0975, Customer Service:(214) 645-7057 or Toll free: (877)-887-8136
Turn Around Time1-3 days
Performed Sunday - Saturday
Performed by Cytogenetics
Notes Conventional cytogenetic analysis should be performed in addition to FISH at initial diagnosis.
Updated/Reviewed 12/12/2016