Veripath Test Directory

Synonyms Aneuploidy FISH
FISH, trisomy panel
Trisomy panel FISH
Description Fluorescence In Situ Hybridization (F SH) is performed to evaluate the number of signals for chromosomes 13, 18, 21, X and Y In each cell. Useful as a rapid screen for aneuploidy on amniotic fluid or blood from a critically Ill newborn. Result must be confirmed by routine chromosome analysis
Specimen Requirements 
Container Sterile tissue culture tube
Specimen Type Amniotic fluid
Preferred Collection Volume 16-20 mL
Minimum Collection Volume 15 mL
Collection Instructions Aseptically handled amniotic fluid in a tightly capped, sterile tissue culture tube. Seal tightly with parafilm. For pregnancies 16 weeks or more, 20 mL fluid should be submitted. Less may be submitted for pregnancies 12-15 weeks.
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over the weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, clotted, hemolyzed or grossly contaminated specimens.
Methodology Fluorescent labeled DNA probes specific for the centromeres of chromosomes X (DXZ1), Y (DYZ3), and 18 (D18Z1), and for chromosome bands 13q14 (RB1) and 21q22.13-q22.2 (D21S259, D21S341, D21S342) are hybridized to unstained interphase nuclei on a slide. 100 cells are scored for the number of each fluorescent signal.
Reference Range See interpretive report.
CPT Code(s) 88271x5, 88275x2, 88291
Department Cytogenetics
Contact Information Cytogenetics Lab: (214) 648-0975, Customer Service: (214) 645 7057 or• Toll Free (877) 887-8136
Turn Around Time1-2 days
Performed Sunday - Saturday
Performed by Cytogenetics
Notes Routine chromosome analysis must be ordered on the same specimen.
Updated/Reviewed 12/12/2016