Veripath Test Directory

Epic Test Name FISH, RB1
Components
Synonyms FISH, Retinoblastoma gene
RB1 FISH
Description Fluorescence In situ hybridization (FISH) is performed to detect deletion of the RB1 gene as seen In chronic lymphocytic leukemia (CLL) and multiple myeloma.
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Bone marrow, blood, tissue
Preferred Collection Volume 2 mL bone marrow, 5-10 mL blood, 5 mm3 tissue
Minimum Collection Volume
Collection Instructions 1. Bone Marrow: 2 mL aspirated bone marrow preferred) anticoagulated with preservative-free sodium heparin (e.g., greenĀ­ top tube). DO NOT use lithium heparin. Bone marrow biopsy may be submitted if aspirate is not available - see instructions for
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. Frozen or fixed tissue specimens, or tissue specimens sent without tissue culture medium.
Methodology A fluorescent labeled DNA probe for the RB1 gene, at chromosome band
13q14, Is hybridized to unstained interphase nuclei on a slide. 200 cells are evaluated to determine whether two signals are present (no deletion) or fewer than 2 signals are present (deletion).
Reference Range
CPT Code(s) 88271; 88275; 88291
Department Cytogenetics
Contact Information (214) 648-0975 or (214) 645-7057 or Toll free: (877)-887-8136
Turn Around Time1-3 days
Performed Sunday - Saturday
Performed by Cytogenetics
Link
Notes See also Multiple Mveloma FISH panel and CLL FISH panel.

Conventional cytogenetic analysis should be performed in addition to FISH at initial diagnosis
Updated/Reviewed 12/12/2016


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