Veripath Test Directory

Epic Test Name FISH, MLL GENE REARRANGEMENTS
Components
Synonyms 11q23 FISH
MLL FISH
Description Fluorescence In situ hybridization (F SH) is performed to detect rearrangements of the MLL gene, observed in acute myeloid leukemia FAB type M4 and MS, acute lymphoblastic leukemia, and following treatment with topolsomerase II inhibitors.
Specimen Requirements 
Container Green top (sodium heparin) collection tube
Specimen Type Bone marrow, blood, tissue
Preferred Collection Volume 2 mL bone marrow, 5-10 ml blood, 5mm³ tissue
Minimum Collection Volume
Collection Instructions 1. Bone Marrow: 2 mL aspirated bone marrow preferred) anticoagulated with preservative-free sodium heparin (e.g., green­ top tube). DO NOT use lithium heparin. Bone marrow biopsy may be submitted if aspirate is not available - see instructions for
Specimen Handling Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
Rejection Criteria
Methodology A dual color fluorescent labeled DNA probe for the MLL gene, at chromosome band llq23,is hybridized to unstained Interphase nuclei on a slide. 200 cells are evaluated to determine whether the signals are fused (normal) or separated (rearrangement present).
Reference Range
CPT Code(s) 88271x2; 88275; 88291
Department Cytogenetics
Contact Information Lab: (214) 648-0975, Cutomer service:(214) 645-7057, or Toll free: (877)-887-8136
Turn Around Time1-3 days
Performed Sunday - Saturday
Performed by Cytogenetis
Link
Notes See also MDS FISH panel

Conventional cytogenetic analysis should be performed In addition to FISH at initial diagnosis.
Updated/Reviewed 12/12/2016


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