Veripath Test Directory

Epic Test Name CULTURE AFB
Synonyms Acid Fast Bacilli, Mycobacteria, TB
Culture, TB (Tuberculosis), AFB (Acid-fast bacilli)
Description Gold standard test for diagnosing the presence of mycobacterial organisms.
Specimen Requirements 
Container Sterile plastic container (leakproof) 2-8 C.
Specimen Type Collect one of the following: Sputum, CSF, urine, body fluid, tissue, gastric aspirate, or stool.
Preferred Collection Volume Sputum: 10-15 mL; CSF: 5 mL; urine >/= 40mL; body fluid: >/= 5 mL; tissue: 1 gram: gastric aspirate: 5-10mL.
Minimum Collection Volume Sputum: 1 mL; CSF: 1 mL; urine 10 mL; body fluid: 1 mL; tissue visible; gastric aspirate: 1 mL.
Collection Instructions Collection of early morning specimens of urine and sputum. 1 Sputum per 24 hrs. Collection of 3 morning sputum samples with >24hrs in between optimal.
Specimen Handling Stability (collection to initiation of testing): Ambient: 24 hrs; refrigerated: 1 week; frozen: 1 week.
Rejection Criteria Dry material or material collected and transported on a swab.
Methodology Stain/Culture/Identification/Susceptibility
Reference Range Culture negative for acid fast bacilli.
CPT Code(s) 87116
Department Microbiology
Contact Information Ph: 214-633-4940
Turn Around TimeStain: 24 hrs; Positive: reported as soon as detected;Final: Negative after 6 weeks
Performed Daily
Performed by Microbiology
Notes Cultures performed until 3 positive specimens obtained. After 3 positive samples, testing is limited to AFB smear only. Call Microbiology at ext 55040 if an exception is requested. Includes AFB smear.
Updated/Reviewed 02/22/2018