Veripath Test Directory

Synonyms Activated Protein C Resistance, APCR, APC Resistance, APC·R
Description Activated Protein C Resistance is a simple functional clotting test sytem intended for screening individuals for Factor V Leiden and other simular Factor V mutations. Activated Protein C Resistance is associated with a point mutation in the Factor V gene (e.g., Factor V Leiden). Activated Protein C resistance is determined by measuring the prolognation of clotting time in plasma in response to activated Protein C. In the test, the patient's PTT is detemined in the presence and absence of activated Protein C. The result is expressed as the ratio of the two clotting times. Unlike the test for Factor V Leiden by PCR, Activated Protein C Resistance test is a screening test that will detect all Factor V defects that result in Protein C Resistance (e.g. Factor V Hongkong, Factor V Cambridge, etc.)
Specimen Requirements 
Container Light blue ( 3.2% sodium citrate) collection tube
Specimen Type Blood
Preferred Collection Volume 3 mL
Minimum Collection Volume Adult: 2 mL, Neonates : 2 mL
Collection Instructions When drawing specimens for coagulation testing, always draw a "pilot" tube (7-10ml plain red top tube), completely filled. This removes tissue fluid contamination. Samples should be obtained from peripheral vein wheneever possible. If drawn from indwelling catheter, the line should be flushed with 15mL of saline. After irrigation, a "pilot" tube should be drawn and discarded.
Specimen Handling In-house patients, transport at room temperature.
Out-side client, follow collection instructions and the steps below:
1. Spin light blue top tube. Remove plasma avoiding platelet/buffy coat layer. Spin plasma again to remove top portion of plasma ans place 1mL of PLATELET-POOR PLASMA in plastic tube. (Glass tube unacceptable). Double-centrifugation is critical for accurate results as platelet contamination may cause erroneous results.
2. Plasma must be frozen within 4 hours of collection. CRITICAL WARNING: Specimens must remain frozen until ready to test. Submit separate aliquots when multiple tests are ordered on the same patient specimen.
Rejection Criteria Hemolysis, clotted, short sample, >4 hrs old (if not frozen), thawed plasma.
Methodology Clotting based assay
Reference Range See interpretive report.
CPT Code(s) 85307
Department Hemostasis/Coagulation
Contact Information Hemostasis Lab; 214-633-4959 Customer Service: 214-633-0400, Toll Free 1-877-887-8136.
Turn Around Time1-7 days
Performed Wednesday
Performed by Hemostasis Lab
Updated/Reviewed 07/23/2016