Veripath Test Directory

Epic Test Name ADAMTS-13 Evaluation
Components
Synonyms ADAMTS-13
Description ADAMTS-13 is a plasma enzyme that is responsible for cleaving of unusually large vonWillebrand multimers into smaller normal fragments. Absent or low ADAMTS-13 activity allows formation of platetlets micthrombi in small vessels due to presistence of ultra large vonWillebrand multimers, generating the clinical sequelae of TTP. ADAMTS-13 activity is measured by Fluorescence Rensonance Energy Transfer(FRET) with a synthetic substrate. ADAMTS-13 inhibitor is determined by using mixing studies. The ADAMTS-13 antibody assay detects IgG antibody seroloically by ELISA. ADAMTS-13 a reflexive testing algorithm. Activity is always performed. If activity result is ≤ 30%, the inhibitor assay will be perfomred. If inhibitor result is ≤ 0.7 inibitor units, the antibdoy assay will be performed.
Specimen Requirements 
Container 3 mL Light blue (3.2% sodium citrate) collection tube
Specimen Type Blood
Preferred Collection Volume 3 mL
Minimum Collection Volume 2 mL
Collection Instructions Completely fill the Blue Top (sodium citrate) tube. Underfilled tubes will not be accepted. Mix sample immediately by gentle inversion at least six times.
Specimen Handling Outpatient specimens: Specimens should be centrifuged and frozen within 4 hours of collection to generate platelet-poor plasma. A minimum of 0.5 ml frozen citrated plasma is needed per test ordered. Inpatient specimens: Transport at room temperature. Specimens should be received in lab within 2 hours of collection.
Rejection Criteria Hemolysis, clotted, short sample, >4 hrs old (if not frozen), EDTA Tube.
Methodology FRET, Mixing Studies, ELISA
Reference Range
CPT Code(s)
Department Hemostasis/Coagulation
Contact Information Hemostasis Lab; 214-633-4959 Customer Service: 214-633-0400, Toll Free 1-877-887-8136.
Turn Around Time3-7 days
Performed Monday - Friday
Performed by Blood Center of Wisconsin
Link https://www.bcw.edu/bcw/Diagnostics/Testing-Services/test-catalog/detail/index.htm?title=ADAMTS13_Evaluation&OrderCode=1295&search=1295
Notes
Updated/Reviewed 05/01/2019


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