Veripath Test Directory

Epic Test Name MTHFR MUTATIONS (C677T and A1298C)
Synonyms MTHFR mutations (Ala222Val, Glu429Ala)
MTHFR ther molabile polymorphisms
Methyelenetetrahydrofolate reductase DNA variant analysis
Specimen Requirements 
Container Lavender (EDTA) collection tube
Specimen Type Peripheral blood
Preferred Collection Volume 1 mL
Minimum Collection Volume 0.5 mL
Collection Instructions Peripheral Blood: 1 ml whole blood in EDTA preferred. ACD is acceptable. Send specimen at room temperature or with cold pack.
Specimen Handling Specimen must arrive within 72 hrs of collection (48 hours for ACD).
Rejection Criteria Clotted peripheral blood. Specimens drawn in heparin will be rejected due to heparin's inhibitory nature to Taq Polymerase.
Methodology PCR, cleavase, FRET (Hologic)
Reference Range Wild Type
CPT Code(s) 81291
Department Molecular Diagnostics
Contact Information Lab: (214)-648-0960
Turn Around Time1-5 days
Performed Twice weekly
Performed by Molecular Diagnostics
Updated/Reviewed 12/07/2016