Veripath Test Directory

Epic Test Name B-CELL CLONALITY PCR
Components
Synonyms B-cell Ig Heavy Chain PCR
JH gene rearrangement
B cell gene rearrangement
J-Heavy Chain PCR (framework 1,2,3a)
Immunoglobulin heavy chain gene rearrangement
Description
Specimen Requirements 
Container Lavender (EDTA) collection tube
Specimen Type Peripheral blood, bone marrow, tissue, paraffin block
Preferred Collection Volume 1 mL
Minimum Collection Volume 0.5 mL
Collection Instructions For tissue: 5x5x5 mm or 500 mg of tissue. Tissue must be frozen within 1 hr of collection. Send on dry ice or 5 FFPE slides @ 10-micron each
Specimen Handling Specimens must arrive within 72 hrs of collection unless FFPE. If available, a hematopathology report and H&E slide should accompany the specimen.
Peripheral Blood: 1 mL blood in EDTA. Send specimen at room temperature or with cold pack.
Bone Marrow: 1 mL blood in EDTA. Send specimen at room temperature or with cold pack.
Tissue: 5x5x5 mm or 500 mg of tissue, frozen within 1 hour of collection. Send on dry ice.
Paraffin Block or Unstained Slides: Formalin fixed only; not B-5 fixed

Rejection Criteria B5 fixed tissue. Clotted peripheral blood or bone marrow. Thawed tissue specimen. Specimens drawn in heparin will be rejected due to heparin's inhibitory nature to Taq Polymerase.
Methodology Polymerase Chain Reaction
Reference Range Not Detected
CPT Code(s) 81261, (88381 or 88387)
Department Molecular Diagnostics
Contact Information Lab: (214)-648-0960
Turn Around Time< or = 5 days from receipt of specimen
Performed As requested
Performed by Molecular Diagnostics
Link
Notes
Updated/Reviewed 12/07/2016


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