Veripath Test Directory

Epic Test Name T&B SUBSET QUANT w/NKs (CD3/CD4/CD8/CD19/CD16+CD56)
Synonyms Immunodeficiency evaluation and monitoring; Quantitative lymphocyte analysis-lymphocyte subsets; Lymphocyte subsets; T-cell subsets (helper/suppressor ratio); CD4 count; CD3 count; T,B, NK subset quantification; Extended lymphocyte subset panel
Description These are a set of quantitative tests used to determine the aboslute numbers of various subsets of lymphocytes in the blood (e.g., total T cells, CD4 T cells, CD8 T cells, B cells and NK cells). The test can be ordered as a whole (i.e., T/B/NK) or specific subsets can be ordered (i.e., T, B or NK). For example, in HIV disease, only a CD4 count is typically monitored. In a workup for primary immunodeficiency, the physician may request an Extended Lymphocyte Subset panel, which provides a more detailed characterization of the lymphocyte subsets.
Specimen Requirements 
Container Lavender (EDTA) collection tube
Specimen Type Peripheral blood
Preferred Collection Volume 4 mL Lavender (EDTA)
Minimum Collection Volume 2 mL Lavender (EDTA)
Collection Instructions Specimens cannot be frozen or placed in any fixative (i.e. formalin). Transport peripheral blood at room temperature.

Please submit WBC count & Percent (%) Lymphs from CBC report from the same drawn as the sample being tested.
Specimen Handling Specimens cannot be frozen or placed in any fixative (i.e. formalin).
To process the specimen, the request form must include:
* Collection date and time.
* Pertinent clinical history (with relevant ICD-9 codes, if available).
* Specimen source (fluid type, tissue origin).
* Patient identification (i.e. name, DOB, MR#).
* Physician requesting test.
Rejection Criteria Tube less than 1/2 full, specimen clotted, sample severely hemolyzed, frozen. Specimen Stability: K2 - K3 EDTA specimen should be processed within 48 hours of collection.
Methodology Flow Cytometry, Dual Platform
(CD3/CD4/CD8/CD45) and/or (CD3/CD16 CD56/CD45/CD19)
Reference Range Ranges based on a study that evaluated a large number of healthy donors (Shearer, et al, J. Allergy Clin Immunol, 2003, 112(5):972-80). Adult (>18 years) reference ranges were developed in-lab by the clinical Laboratory Services for Flow Cytometry at UT Southwestern Medical Center.
CPT Code(s) CD3 quantification: 86359
CD4 quantification: 86361
T cell subset: 86359, 86361
T cell and B cell subset: 86359, 86360, 86355, 86357
B cell subset: 86355 and 86357
NK cell subset 86357
Department Flow Cytometry
Contact Information 214-648-0930 or 214-633-5227
Turn Around TimeResults available next business day. Same day results are available if the sample is received in the LAB before 12 NOON, Monday-Friday
Performed Monday - Saturday
Performed by Flow Cytometry
Notes Please submit WBC count and Percent (%) Lymphs from CBC report from the SAME draw as the sample being sent for testing.
Updated/Reviewed 11/08/2018