Veripath Test Directory

Epic Test Name CHROMOSOME ANALYSIS, NON-CANC, PRENATAL
Components
Synonyms Cytogenetics, Amniotic Fluid
Karyotype, Amniotic Fluid
Description Cytogenetic analysis of amniocytes for prenatal diagnosis of chromosome abnormalities.
Specimen Requirements 
Container Sterile container
Specimen Type Amniotic fluid
Preferred Collection Volume 16-20 mL
Minimum Collection Volume 15 mL
Collection Instructions Aseptically handled amniotic fluid in a tightly capped, sterile tube. Seal tightly with parafilm. For pregnancies 16 weeks or more, 20 mL of fluid should be submitted. Less may be submitted for pregnancies of 12-15 weeks.

Note: Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported Immediately. Do not freeze. Protect from heat or cold.
Specimen Handling Deliver to lab at room temperature within 24 hrs. If collected over the weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately.
Rejection Criteria Frozen, fixed or grossly contaminated specimen.
Methodology Amniotic fluid is cultured in four to six vessels, then harvested In situ and G-banded with Trypsin- Wright stain. The chromosomes in fifteen metaphase spreads from fifteen colonies in at least 2 separate cultures are counted. Five cells are fully analyzed, and two are karyotyped.
Reference Range
CPT Code(s) 88235, 88269, 88280, 88291
Department Cytogenetics
Contact Information Cytogenetics Lab: (214) 648-0975, Customer Service: (214) 645-7057 or Toll Free (877) 887-8136
Turn Around Time7-10 days
Performed Sunday - Saturday
Performed by Cytogenetics
Link
Notes Five cells are fully analyzed, and two are karyotyped.
Updated/Reviewed 10/28/2015


Label