Veripath Test Directory

Epic Test Name FISH, Her2/neu
Synonyms Her2 FISH, Her2/neu FISH
Description Fluorescence in situ hybridization (FISH) is performed to detect the Her2/neu gene amplification, most commonly observed in breast cancer and lung cancer.
Specimen Requirements 
Container Two unstained positively charged slides cut at 4 microns and one H&E stain slide.
Specimen Type FFPE (Formalin Fixed Paraffin Embedded Tissue)
Preferred Collection Volume
Minimum Collection Volume
Collection Instructions Specimens subject to HER2 (ERBB2) testing should be fixed in 10% neutral buffered formalin for at least six hours and up to 72 hours. The volume of formalin should be at least 10 times the volume of the specimen. Decalcification solutions with strong acids should not be used.
Specimen Handling
Rejection Criteria Cases without H&E slide.
Methodology A dual color fluorescent labeled DNA probe for the Her2/neu gene, is hybridized to unstained interphase nuclei on a slide. 50 cells are evaluated to determine whether the signals are amplified.
Reference Range See interpretive report.
CPT Code(s) 88366
Department Cytogenetics
Contact Information Cytogenetics Lab: (214) 648-0975, Customer Service: (214) 645-7057 or Toll Free (877) 887-8136
Turn Around Time6 working days after specimen arrival in Cytogenetics Lab
Performed 3 times a week
Performed by Cytogenetics
Notes Fixation guidelines for clients:
1. Specimens should be immersed in fixative within one hour of the biopsy or resection.
2. If delivery of a resection specimen to the pathology department is delayed (e.g. specimens from remote sites), the tumor should be bisected prior to immersion in fixative. In such cases, surgeons must ensure that the identity of the resection margins is retained in the bisected specimen; alternatively, the margins may be separately submitted.
3. The time of removal of the tissue and the time of immersion of the tissue in fixative should be recorded and submitted to the laboratory.
Updated/Reviewed 12/12/2016